Figure 2
From: “High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
Characterization of tissue-specific expression and derivation of “regions of interest”. (A) Median profiles derived from various C. elegans transgenic lines expressing a fluorescent marker in each respective tissue. Animals were concurrently live-stained with DiO or DiI (which labels sensory neurons) to enable consistent orientation across strains. (B) Graphical representation of transgenic lines in (A) to demonstrate resolution of individual tissues as a function of the animal’s length. (C) Difference (Δ) plot (top) derived from average profiles in (A) and significance plot (bottom) based on these differences. For significance plots “−1.3-log10(P-value)” was plotted which distinguished regions of significance by Wilcoxon Rank Sum Test. A value ≥ 0 is equilvalent to P ≤ 0.05. (D) Anatomical representation of various C. elegans tissues and the derived “regions of interest” from them (e.g. Anterior: 0–20%, Middle: 20–85%, and Posterior: 85–100%). Pharynx in red, neurons in orange, muscle in green, and intestine in grey. (E) Simplified schematic of these three regions, Anterior (white), Middle (grey), and Posterior (dark grey) to demonstrate the relative cutoff points of these various areas of interest. All animals are positioned with the anterior side to the left. Plots are representative of three biological replicates and derived from n ≥ 50 organisms. Sample size (n) for all experiments and exact P-values can be found in the Supplementary Table. L.A. Daniele provided the illustrations.
