Figure 7
From: “High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
Mitochondrial morphology screening after treating C. elegans with selected RNAi’s. (A) Mitochondrial mass after selected RNAi knockdown of mitochondrial morphology genes. Muscle-specific, mitochondria-localized MLS2::GFP = “mito::GFP”. “n.s.” = not significant, ***P < 0.001, and “Ω” = P < 0.00001. (B) Median profiles of mitochondrial MLS2::GFP after RNAi treatments. (C) Significance (log10(P-value)) plots derived from median profiles in (B) compared to “empty vector” (control). Asterisks (*) = regions that deviate significantly from controls. For significance plots, a value ≥ 0 is equivalent to P ≤ 0.05 by Wilcoxon Rank Sum Test. Plots are representative of two biological replicates of n ≥ 100 organisms. High magnification micrographs of muscle-specific, matrix-localized MLS2::GFP signal, across a representative animal for control (empty vector, black boxes), immp-1 RNAi (blue boxes), mfn-1 RNAi (yellow box), and W108.5 RNAi (red boxes). Scale bar is 10 µm. Muscle cell nuclei = “n”. (D) Mitochondrial membrane potential, Δψ (by JC-9 ratio) after RNAi treatments in (A–C). ***P < 0.001, and “Ω” = P ≤ 0.00001. (E) Significance (log10(P-value)) plots derived from median Δψ comparing each profile to “empty vector” treated “controls. Asterisks (*) = identified sites in (C) where Δψ is also affected. White arrowheads = positions where both mitochondrial morphology (in C) and Δψ are affected. Plots in (D,E) are representative of four biological replicates of n ≥ 100 organisms. Sample size (n) for all experiments and exact P-values for Wilcoxon Rank Sum tests against empty vector “control” can be found in the Supplementary Table. L.A. Daniele provided the illustrations.
