Figure 3
From: A haplotype in CFH family genes confers high risk of rare glomerular nephropathies
Three-dimensional models of CFHR3WT, CFHR3Arg142Cys and CFHR3/C3b complex. (A) Models of WT and MUT CFHR3 obtained by homology-modeled and shown with 5 complement control protein modules (CCPs) by DS 3.0. The β-strands are shown in turquoise, а-helices in red, and the loops connecting in WhiteSmoke™. The location of the Arg142 and Cys142 is highlighted in orchid. (B) Generated models show the discrimination of local intramolecular hydrogen bonding interactions between Arg142 and Cys142 by MOE. Hydrogen bonds are shown with a fluorescent green dotted line representation. (C) Structure of CFHR3 in complex with the ligand C3b were generated with the DS 3.0. CFHR3 is denoted by pink; C3b (based on the C3 crystal structure, PDB 2WII) consists of a β-chain (residues 1–642) shown in turquoise and α’-chain (residues 730–1641) in steel blue. The binding regions of the CFHR3/C3b complex are presented in yellow. The Arg residue 142 of CFHR3 is highlighted with a CPK model and shown to be directly involved in the binding to C3b.
