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Figure 1

From: Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells

Figure 1

Growth and differentiation of MSCs in HPL and FCS. (a) Phase contrast images of MSCs (passage 2) that were in parallel cultivated in HPL and FCS. (b) Population doublings (PDs) within the first two passages were compared in HPL-MSCs and FCS-MSCs (n = 6). PDs in passage zero are not considered due to lack of initial cell numbers. (c) Average doubling time during passage 1 and 2 was shorter in HPL than FCS (**p < 0.01). (d) The time from initial isolation to passage two was shorter in HPL than in FCS (**p < 0.01). (e) Histograms depict the immunophenotype of MSCs isolated in FCS or HPL (autofluorescence in black). (f) The percentages of positive MSCs for the immunophenotypic markers did not differ in FCS or HPL. (g) HPL-MSCs and FCS-MSCs at passage 2 were in parallel induced with the same differentiation media for three weeks towards adipogenic or osteogenic lineage, and then stained for fat droplets or calcium precipitates (BODIPY/DAPI and alizarin red, respectively).

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