Figure 1
From: Identification of FBXL4 as a Metastasis Associated Gene in Prostate Cancer
Common loss of 6q16 including FBXL4 locus is present in prostate cancer samples. (a) Minimum overlapping region of copy number loss at 6q16.1–16.2 (blue box) in six fresh frozen prostate cancer bone metastases detected by Affymetrix SNP array 6.0. Heterozygous deletion is present in all six samples. SNPs with normal copy number are depicted in blue, loss in green and gain in red. (b) SNP array results were confirmed with copy number analysis of 6q16.1–16.2 at FBXL4 locus by TaqMan DNA copy number assay. Heterozygous FBXL4 loss was confirmed in 5/6 samples. Sample 3 (Met 3) with least obvious deletion on SNP array shows close to normal FBXL4 copy number by qPCR, indicating a small proportion of cells with this deletion. Error bars: SD; n = 3. (c) Representative FISH images of FBXL4 region in prostate clinical samples. FBXL4 copy number loss in a metastatic and primary FFPE prostate cancer sample is visible in most nuclei (blue) as a reduction in number of red signals (FBXL4) in relation to green (control) signals. Neutral copy number in a BPH case is reflected by equal number of red (FBXL4) and green (control) signals per nucleus. Scale bar: 10 µm.
