Figure 1

TGF-β2 stimulates Fgf23 gene expression and FGF23 protein synthesis in UMR106 cells. Arithmetic means ± SEM (n = 7) of (A) Fgf23 mRNA abundance (relative to Tbp mRNA) and (B) FGF23 (C-terminal) protein concentration (pg/ml) in the cell culture medium of UMR106 cells incubated without (ctrl, white bars) or with TGF-β2 (black bars, 10 ng/ml, 24 h). **p < 0.01, ***p < 0.001 indicate significant difference compared to UMR106 cells treated with vehicle only (paired t-test). (C) Arithmetic mean ± SEM (n = 5) of Fgf23 mRNA levels (relative to Tbp mRNA) in UMR106 cells after 24-hour treatment without (ctrl, white bar) or with TGF-β2 (0.3–10 ng/ml, black bars). ***p < 0.001 indicates significant difference compared to UMR106 cells treated with vehicle only (ANOVA).