Figure 1
PAQR-1 depletion augments survival of ER stressed animals. (A) Eggs derived from WT, paqr-1(tm3262), paqr-2(tm3410) and paqr-3(ok2229) strains were laid on plates in the absence (white bars) or presence of 5 μg/ml tunicamycin (black bars). Percentage of eggs that developed into mature adults was scored. Each strain was scored on four independent plates and each experiment was repeated independently at least three times. Error bars represent SEM for repeat plates within the experiment. ***P < 0.001 vs. WT + tunicamycin. (B) Survival of eggs that developed into mature adults after 8 days on tunicamycin (5 μg/ml). Representative images illustrating survival of WT and paqr-1(tm3262) strains are shown. Differences on bacterial lawn thickness at the time of observation indicate changes in mobility and viability. Scale bar, 250 μm. White arrowhead indicates a dead worm; white arrows indicate eggs that never developed into mature adults; red arrowheads indicate live worms; red arrows indicate newly hatched eggs. (C) WT (black), paqr-1(tm3262) (red), paqr-2(tm3410) (blue), and paqr-3(ok2229) (green) animals were transferred at day 1 or day 7 onto plates containing 50 μg/ml tunicamycin and survival was monitored. Lifespan values are given in Table S1.
