Figure 6

The expression levels of TNFSF10, TCONS_00158758 and IFN-β. Expression levels were normalized to GAPDH. Data are the means ± S.D. of three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05, one-way analysis of variance followed by Dunnett’s multiple comparison test or Student’s t-test was performed. (a) and (b). PAM cells were mock infected or infected with GSWW, FL-12 and VR2332 (at the MOI of 1). Total RNA was extracted 24 hpi and the first-strand cDNA was synthesized. RT-qPCR experiments were performed to detect TNFSF10 expression. (c) RT-qPCR results showing fold change in lncRNA TCONS_00158758 expression level in PAM cells at 24 hpi with gradient GSWW infection. A total of 10⁷ cells were plated in 25 cm² flasks and mock infected or infected with 0.5 MOI, 0.1 MOI, 0.02 MOI, 0.004 MOI of GSWW per flask. (d) RT-qPCR data of lncRNA expression in PAM cells that were mock infected or infected with live GSWW, and VR2332 (at the MOI of 0.1) or heat-inactivated (56 °C, 120 min) viruses at 24 hpi. (e) PAM cells were treated with 100 µg/ml poly (I:C) for 24 hours. Total RNA was extracted and the first-strand cDNA was synthesized. RT-qPCR experiments were performed to detect the expression of IFN-β and lncRNA TCONS_00054158. (f) and (g) PK-15 cells were transfected with 2 µg/ml poly (I:C) for 24 hours. Total RNA was extracted and the first strand cDNA was synthesized. RT-qPCR experiments were performed to detect the expression of IFN-β and lncRNA TCONS_00054158. (h) and (i). PK-15 cells were treated with 100 µg/ml poly (I:C) for 24 hours. Total RNA was extracted and the first strand cDNA was synthesized. RT-qPCR experiments were performed to detect the expression of IFN-β and lncRNA TCONS_00054158.