Figure 1
From: Ammonium chloride alters neuronal excitability and synaptic vesicle release
Effects of ammonium chloride (NH4Cl) on passive membrane properties. (A) Sample traces of resting membrane potential (Em) recorded continuously in current-clamped neurons in the presence of synaptic blockers and TTX with increasing concentrations of NH4Cl (5–10–50 mM). (B) Changes of Em in neurons exposed to NH4Cl. (C) Averaged Ems in response to NH4Cl, all of which were measured at 5 minutes after the applications. Only 50 mM caused slow and reversible depolarization (to ~−35 mV, n = 8 neurons, p < 0.01, F = 20.28, one-way ANOVA). (D) None of the NH4Cl treatments changed the input resistance (Rin) of the recorded neurons (n = 8 neurons, p = 0.43, F = 0.98, one-way ANOVA).
