Figure 8
From: Dynamic microtubule association of Doublecortin X (DCX) is regulated by its C-terminus
Hyperosmotic stress slowing of tubulin dynamic change is further slowed by DCX WT but not DCX ΔC. (A–C) The recovery of the ROI fluorescence for GFP-α-tubulin only and together with myc-DCX WT or myc-DCX ΔC under sorbitol treatment conditions. Results are for the mean ± SEM for (A) the initial rate of recovery of fluorescence for GFP-α-tubulin only or together with myc-DCX WT or myc-DCX ΔC under sorbitol treatment conditions in the photobleached area (estimated over the initial 6 s post-bleach), (B) the time to reach half-maximal recovery of fluorescence (t1/2), and (C) the fluorescence maximum recovery for GFP-α-tubulin in the bleached area. Error bars represent the standard error of the means and asterisks indicate values calculated to statistically significantly different **p ≤ 0.01, ****p ≤ 0.0001, n = 10 cells in each of three independent experiments. n.s. = not significant).
