Figure 3 | Scientific Reports

Figure 3

From: 5-LO inhibition ameliorates palmitic acid-induced ER stress, oxidative stress and insulin resistance via AMPK activation in murine myotubes

Figure 3

Effects of 5-LO siRNA on PA-induced lipotoxicity. C2C12 myotubes were transfected with negative control siRNA or 5-LO siRNA (20 nM), and then treated with PA (750 μM) for 12 h (qPCR) or 24 h (western blot, ELISA, ROS). The effects of 5-LO knockdown were determined by western blotting (a). Levels of LTB4 in culture media were measured by ELISA (b). The effects of 5-LO siRNA on the mRNA levels of ER stress markers were determined by qPCR (c). ROS production was assessed using AmplexRed (d). Levels of pro-inflammatory cytokines were determined by ELISA (e). Akt and serIRS-1 phosphorylation was assessed by western blotting (f). The results of the western blotting are shown as the representative of three independent experiments (f), and other results are expressed as means ± SDs of three experiments performed in triplicate. # P < 0.05 vs. non-treated control siRNA, *P < 0.05 vs. PA-treated control siRNA.

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