Figure 5
From: Dopamine 2 Receptor Activation Entrains Circadian Clocks in Mouse Retinal Pigment Epithelium
DA did not induce phase-shifts in the RPE-choroid of PER2::LUC bioluminescence rhythms in D2R−/−PER2::LUC mice. Representative example shows that DA treatment at CT 24 did not phase-shift D2R−/−PER2::LUC bioluminescence rhythm (A). The blue trace indicates a control (Veh treated) and red trace indicates a DA treated RPE-choroid culture. The black arrow indicates time of the drug or vehicle treatment (A). DA treated RPE-choroid cultures at CT 5–9 and at CT 21–24 were averaged for statistical analysis. Data were used to calculate the phase change of D2R−/− versus controls. Bars show the mean amount of phase change from controls and error bars show ±SEM for experimental groups. Error bars from x axis show ±SEM for control groups. No phase-shifts were observed in the D2R−/−PER2::LUC RPE-choroid rhythms at either time points (B) t-test, n = 6 for DA and Veh for each time point).
