Figure 4 | Scientific Reports

Figure 4

From: Epigenetics of the preferential silencing of Brachypodium stacei-originated 35S rDNA loci in the allotetraploid grass Brachypodium hybridum

Figure 4

Immunopatterns of H4K5ac in the nuclei isolated from B. hybridum roots. H4K5ac foci distribution in nuclei with one (a1–a4), two (b1–b4) or none (c1–c4) chromocentre(s) adjacent to the nucleolus. a1,b1,c1–DAPI counterstained nuclei; a2,b2,c2–H4K5ac foci distribution; a3,b3,c3–superimposed channels H4K5ac foci distribution (red) and DAPI (blue); a4,b4,c4–schematic representation of H4K5ac distribution in the nucleolus and adjacent chromocentres. (d1) FISH with 25S rDNA (red) as a probe in B. hybridum nucleus. (d2) H4K5ac immunostaining pattern in nucleus presented on (d1). The locations of B. stacei-originated 35S rDNA loci are indicated by yellow insets. (d3) Schematic representation of H4K5ac foci distribution in the 35S rDNA loci superimposed from B. distachyon (Bd’; Bd”) and B. stacei (Bs’; Bs”) in B. hybridum nucleus. Nu – nucleolus. Scale bar: 5 µm.

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