Figure 4

dA regulated the expression of mitochondria associated apoptotic proteins. (a) Western blot analysis of mitochondria associated apoptotic proteins expression after dA treatment for different time (3 h, 6 h, 12 h, 24 h). Treating for 0 h was used as the internal standard; (b,c) Expression of phosphorylated p38 (p-p38), as measured by Western blot. Lower panel: densitometry analysis of p-p38 expression (n = 3) *p < 0.05 vs 0 h. Data are expressed as the fold change over p38 (set as 1). *p < 0.05 vs the respective control. (d,e,f) B16F10 cells were incubated with 50 μM dA for 24 h and then treated with 200 μM H₂O₂ for 2 h. (d) Western blot analysis of Bax, PARP, and Caspase-3 expression. GAPDH were used as internal standards (n = 3). (e) Expression of p-p38 after treating with dA and H₂O₂. (f) The fold changes of p-p38 expression over the respective control. The results obtained from 3 independent experiments. *p < 0.05, **p < 0.01 vs control group (0 h). Full-length blots indicated in Fig. 4 were presented in Supplementary Fig. 4.