Figure 2

rGal3C negatively affects viability, migration and invasion of HepG2 cells. (A) The viability of HepG2 cells was inhibited after treated by 10, 50 and 100 μg/mL of rGal3C for 12 or 24 hours. (B) Cell migration was attenuated after stimulated by 50 μg/mL rGal3C for 12 hours. (C) Relative cell migration was attenuated to 59.8 ± 1.5% in HepG2 cells treated by 50 μg/mL rGal3C for 12 hours. Representative images were shown on the left, and the quantification of three replicates was shown. (D) The invasive capacity of HepG2 cells was inhibited after stimulated by by 50 μg/mL rGal3C for 12 hours. (E) The invaded cell munber was decreased (from 264 ± 6 to 93 ± 3) after stimulated by 50 μg/mL rGal3C for 12 hours. The quantification of three replicates was shown. Error bars indicate the mean ± SD. *p < 0.01. Con, untreated HepG2 cells.