Figure 5

Ssu72 regulates the reciprocal balance between Th17 and Treg cells in mice with CIA. (A) Populations of IL-17-, CD25-, and Foxp3-producing CD4⁺ T cells were analyzed by intracellular flow cytometry. (B) Spleens from mice with CIA (mock or Ssu72-overexpressing) were subjected to immunostaining to detect the CD4⁺IL-17⁺, CD4⁺CD25⁺FOXP3⁺ and CD4⁺Ssu72⁺ cells (scale bar, 10 μm). Cell numbers were counted in four independent quadrants. The data represent the mean ± SD from three independent experiments. Statistical analyses were conducted using the nonparametric Mann-Whitney U-test (*P < 0.05, **P < 0.03, ***P < 0.01, n = 6).