Figure 5
Impact of pCDs NPs administration on pulmonary homeostasis in mouse. (a) Protocol. Water (Vehicle) or nanoparticles were administered to mice via the endotracheal route during one (1 week tr.) or two weeks (2 week tr.); ♦ = mice euthanasia. (b) Body weight of mice after each treatment. (c) Analysis of the recruitment of immune cells in the lungs parenchyma by flow cytometry. The data represent the number of selected cells on 500,000 events; To define the different cells populations we used the following phenotypes: neutrophils (CD11b+GR1+), eosinophils (F4/80+SiglecF+), CD4 T cells (CD3+CD4+), alveolar macrophages (CD11c+F4/80+) and dendritic cells (CD11b+CD11c+); the Data are mean values ± standard deviation of one representative experiment of two independent experiments; *p < 0.1, **p < 0.01. (d) After treatment, lungs were analysed by histology (H-E staining, scale bar: 200 μm).
