Figure 4

Adenine-induced renal failure in rats is associated with calciprotein ripening and is enhanced by a high phosphate diet. Changes in (a) CrCl, (b) serum urea, (c) serum Ca, (d) serum P, (e) urinary FeP, (f) serum intact PTH, (g) serum intact FGF23 and serum flow cytometric parameters (h) total CPP, (i) CPP SSC-H and (j) CPP-II levels in control and adenine-treated rats fed normal or high-phosphate containing diets over the 10-week study period. *P < 0.05, **P < 0.01, ***P < 0.001, ^‡ P < 0.0001 for adenine-treated groups vs. control groups; ^# P < 0.05, ^## P < 0.01, ^### P ≤ 0.0001 for HPD-fed adenine-treated group vs. NPD-fed adenine-treated group; ^§ P < 0.05, ^§§ P < 0.01 for NPD-fed adenine-treated group vs. NPD-fed control group; ^† P < 0.05 for HPD-fed control group vs. NPD-fed control group. Data presented as mean ± SEM for each experimental group (n = 15 per group). Note logarithmic scale in (g). Ad, adenine; Ca, calcium; Cntrl, control; CPP, calciprotein particles; CrCl, creatinine clearance; FeP, fractional excretion of phosphate; FGF23, fibroblast growth factor 23; HPD, high phosphate diet; NPD, normal phosphate diet; P, phosphate; PTH, parathyroid hormone; SSC-H, side scatter-height.