Figure 3

Slc25a19 knockdown in primary hippocampal neurons significantly reduced TDBTN, TDBL and soma size. (A) Representative images of primary hippocampal neurons transfected with control and Slc25a19 RNAi plasmids, fixed at DIV8, DIV12 or DIV16. Scale bar: 20 μm. (B) Quantitation of TDBTN: Slc25a19 RNAi at DIV8 (1.00 ± 0.04, n.s.), DIV12 (0.57 ± 0.05, P < 0. 001), DIV16 (0.61 ± 0.07, P < 0. 001). (C) Quantitation of TDBL: Slc25a19 RNAi at DIV8 (0.95 ± 0.05, n.s.), DIV12 (0.42 ± 0.02, P < 0. 001), DIV16 (0.39 ± 0.05, P < 0. 001). (D) Quantitation of soma size: Slc25a19 RNAi at DIV8 (0.95 ± 0.03, n.s.), DIV12 (0.77 ± 0.04, P < 0. 001), DIV16 (0.59 ± 0.03, P < 0. 001). (B–D) Two way ANOVA followed by Bonferroni’s post-test. Data for control neurons same as that shown in Fig. 1. (E–G) Significant correlations exist between TDBTN and soma size at DIV8 (E, n = 105, R² = 0.15, P < 0. 001), DIV12 (F, n = 74, R² = 0.34, P < 0. 001) but not DIV16 (G, n = 60, R² = 0.02, n.s.). (H–J) Significant correlations are observed between TDBL and soma size at DIV8 (H, n = 66, R² = 0.24, P < 0. 001), DIV12 (I, n = 74, R² = 0.32, P < 0. 001) and DIV16 (J, n = 60, R² = 0.60, P < 0. 001). In (E–J) Black circles represent control neurons, while purple circles represent Slc25a19 RNAi neurons.