Figure 4

Overexpression of human SLC25A19 significantly rescued the reduction of TDBTN and TDBL induced by Slc25a19 RNAi. (A) Immuostaining of DIV11 neuron transfected with GFP and HA-SLC25A19 constructs. (B) Representative images of DIV10 neurons, conditions as indicated. (C) Quantitation of TDBTN: Ctrl (1.00 ± 0.02), SLC25A19 Ovp (0.91 ± 0.03, n.s. vs Ctrl), Slc25a19 RNAi (0.63 ± 0.02, P < 0.001 vs Ctrl), Slc25a19 RNAi + SLC25A19 Ovp (1.16 ± 0.04, P < 0.001 vs Ctrl, P < 0.001 vs Slc25a19 RNAi). (D) Quantitation of TDBL: Ctrl (1.00 ± 0.03), SLC25A19 Ovp (0.91 ± 0.03, n.s. vs Ctrl), Slc25a19 RNAi (0.58 ± 0.03, P < 0.001 vs Ctrl), Slc25a19 RNAi + SLC25A19 Ovp (0.90 ± 0.04, n.s. vs Ctrl, P < 0.001 vs Slc25a19 RNAi). (E) Quantitation of soma size: Ctrl (1.00 ± 0.02), SLC25A19 Ovp (0.89 ± 0.03, P < 0.01 vs Ctrl), Slc25a19 RNAi (0.85 ± 0.03, P < 0.001 vs Ctrl), Slc25a19 RNAi + SLC25A19 Ovp (0.85 ± 0.02, P < 0.001 vs Ctrl, n.s. vs Slc25a19 RNAi). Data for control neurons same as that shown in Fig. 2. One-way ANOVA followed by Tukey’s post-test. Scale bar: 20 μm.