Figure 5

Slc19a3 knockdown in primary hippocampal neurons significantly reduced TDBTN, TDBL and soma size. (A) Representative images of primary hippocampal neurons transfected with control and Slc19a3 RNAi constructs, fixed at DIV8, DIV12 or DIV16. Scale bar: 20 μm. (B) Quantitation of TDBTN: Slc19a3 RNAi at DIV8 (1.11 ± 0.05, n.s.), DIV12 (0.67 ± 0.03, P < 0. 001), DIV16 (0.32 ± 0.02, P < 0. 001). (C) Quantitation of TDBL: Slc19a3 RNAi at DIV8 (0.98 ± 0.04, n.s.), DIV12 (0.57 ± 0.02, P < 0. 001), DIV16 (0.35 ± 0.02, P < 0. 001). (D) Quantitation of soma size: Slc19a3 RNAi at DIV8 (1.01 ± 0.03, n.s.), DIV12 (0.62 ± 0.02, P < 0. 001), DIV16 (0.57 ± 0.03, P < 0. 001). (B–D) Two way ANOVA followed by Bonferroni’s post-test. (E–G) Significant correlations exist between TDBTN and soma size at DIV8 (E, n = 121, R² = 0.19, P < 0. 001), DIV12 (F, n = 121, R² = 0.37, P < 0. 001) and DIV16 (G, n = 95, R² = 0.57, P < 0. 001). (H–J) Significant correlations are observed between TDBL and soma size at DIV8 (H, n = 121, R² = 0.20, P < 0. 001), DIV12 (I, n = 121, R² = 0.49, P < 0. 001) and DIV16 (J, n = 95, R² = 0.60, P < 0. 001). In (E–J) Black circles represent control neurons, while purple circles represent Slc19a3 RNAi neurons.