Figure 2 | Scientific Reports

Figure 2

From: Resistance exercise initiates mechanistic target of rapamycin (mTOR) translocation and protein complex co-localisation in human skeletal muscle

Figure 2

Rheb, TSC2 and eIF3F peptide competition assays. Immunofluorescence labeling of endogenous proteins was performed in human basal samples (CON – Left panel) as described in the Methods section. Regionality of staining is provided through co-staining with WGA or Dystrophin as a marker of the cell membrane. Competition (COMP) assays were performed to demonstrate antibody specificity of TSC2, eIF3F and Rheb. Non-specific binding/antibody efficiency was examined through imaging samples in which secondary antibody was used without prior primary antibody incubation (No Ab). All image processing was kept constant between images, including exposure time, gain, and image frame. *Significantly different to CON (P < 0.05).

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