Table 1 Primers used in this study.
Gene | Accession number | Sequence (5′-3′) | Location | Expected size (bp) |
|---|---|---|---|---|
Primers for cloning | ||||
p17a (Flag-tagged) | AF330703 | F: CGGAATTCATGCAATGGCTCCGCCATACGA (EcoR I) | 293–314 | 441 |
R: GCTCTAGATCATAGATCGGCGTCAAATCGC (Xba I) | 733–712 | |||
p17b (His-tagged) | AAF45152 | F: CCCAAGCTTATGCAATGGCTCCGCCATACGA (Hind III) | 293–314 | 441 |
R: CCGCTCGAGTCATAGATCGGCGTCAAATCGC (Xho I) | 733–712 | |||
p17 (1–118)a (Flag-tagged) | AF330703 | F: CGGAATTCACAATGCAATGGCTCCGCCATACG (EcoR I) | 293–313 | 354 |
R: AAACTCGAGTCAGGATTGAGACCCGCCATCCCAATG (Xho I) | 646–623 | |||
CDK2c | XM_001113345.3 | F: GCGGAATTCATGGAGAACTTCCAAAAGGTGGAAAAG (EcoRI) | 235–262 | 897 |
R: AAACTCGAGTCAAAGTCCAAGATGGGGTACTGGC (XhoI) | 1131–1110 | |||
Cyclin A2c | NP_001777 | F: AAAGAATTCATGTTGGGCAACTCCGCGCCGGGG (EcoRI) | 306–330 | 1299 |
R: GGGCTCGAGTTACAGATTTAGTGTCTCTGGTGGG (XhoI) | 1604–1580 | |||
σAd | AF104311 | F: TCTAGAACGATGGCGCGTGCCATATAC (XbaI) | 16–33 | 1251 |
R: GTCGACCTAGGCGGTAAAAGTG GC (SalI) | 1266–1249 | |||
PSMB6a | NM_001195714 | F: AAGCTTACCATGGCGGCCACCCTACTAGCT (Hind III) | 19–39 | 697 |
R: GGATCCTCAGGGGGGCGGTAAAGTGGCAAT (Bam HI) | 738–715 | |||
CDK2d | XM_001113345.3 | F: CGGAATTCACCATGGAGAACTTCCAAAAG (EcoRI) | 236–253 | 897 |
R: GTCGACTCAAAGTCCAAGATGGGGTAC (SalI) | 1132–1110 | |||
Primers for semi-quantitative RT-PCR | ||||
RPL 7 | NM_001193556 | F: TCAATGGAGTAAGCCCAAAG | 313–332 | 246 |
R: CAAGAGACCGAGCAATCAAG | 558–540 | |||
RPL 26 | NM_001193566 | F: CATTTCAATGCACCTTCCCAC | 80–100 | 274 |
R: GCCTAGTGATAACCACCTTGC | 331–353 | |||
MDM2 | NM_001266402 | F: GTGAATCTACAGGGACGCCATC | 945–963 | 334 |
R: GGCCCAACATCTGTTGCAA | 1279–1261 | |||
GAPDH | NM_002046 | F: CACCACCATGGAGAAGGCTGGGGCTCA | 480–506 | 454 |
R:GGCAGGTTTCTCCAGACGGCAGGTCAG | 933–907 | |||
