Figure 4
From: Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
This figure demonstrates the impact of nanodomains on the diffusion of fluorescent probes. (A) Two scenarios are possible: the probes diffuse through the nanodomains (case 1); the probes avoid entering the nanodomains (case 2). At sufficiently high domain concentration, the probes diffuse significantly slower as compared to the case where the probes diffuse freely in a homogeneous bilayer32. Panels (B) and (C) show experimental results of two-colour z-scan FCS measurements performed on GUVs. The diffusion coefficients of g-GM1 and DiD are presented as a function of SM content in bilayers of DOPC/SM [(100 − x)/x] (panel B) or DOPC/Chol/SM [(75 − x)/25/x] (panel C). Empty vs. filled symbols mark bilayer mixtures where a homogeneous bilayer vs. a bilayer with nanodomains was detected by FRET. Microscopically phase-separated bilayers are marked by the half-filled symbol. Error bars are the standard deviation within the sample of results (measurements on 5 to 10 different GUVs) obtained for each composition.
