Table 1 The average radius and fractional bilayer area of the nanodomains, distribution constants K D and relative FRET efficiencies E rel (for definition see Materials and Method section) for two different donor-acceptor pairs in DOPC/SM and DOPC/Chol/SM mixtures.
From: Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered
DOPC (mol%) | SM (mol%) | Chol (mol%) | Domain radius (nm) | Domain area (%) | FRET pair | K D(D)** | K D(A)** | E rel*** |
|---|---|---|---|---|---|---|---|---|
100, 75, 70 | 0 | 0, 25, 30 | Homogeneous distrib. | g-GM1/r-GM1 | -----* | -----* | 1.00 | |
95, 92 | 5, 8 | 0 | Homogeneous distrib. | g-GM1/r-GM1 | -----* | -----* | 1.00 | |
90, 88, 85 | 10, 12, 15 | 0 | 8 ± 1 | 37 ± 10 | g-GM1/r-GM1 | ≈10 | ≈10 | 1.03 |
12 ± 3 | 55 ± 10 | |||||||
95, 92, 90, 88 | 5, 8, 10, 12 | 0 | Homogeneous distrib. | CF-PEG-DSPE/Rh-PEG-DSPE | ≈1 | ≈1 | 1.00 | |
70, 67, 65 | 5, 8, 10 | 25 | 9 ± 1 | 45 ± 5 | g-GM1/r-GM1 | ≥20 | ≥20 | 1.12 |
63 | 12 | 25 | Homogeneous distrib. | g-GM1/r-GM1 | -----* | -----* | 1.00 | |
70, 67 | 5, 8 | 25 | Homogeneous distrib. | CF-PEG-DSPE/Rh-PEG-DSPE | ≈1 | ≈1 | 1.00 | |
65, 63 | 10, 12 | 25 | 8 ± 1 | 55 ± 5 | CF-PEG-DSPE/Rh-PEG-DSPE | ≈5 | ≈5 | 1.10 |
60 | 10 | 30 | 9 ± 1 | 45 ± 5 | g-GM1/r-GM1 | ≥20 | ≥20 | 1.12 |
