Figure 3
From: Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
Erythropoietin attenuates the reduction in erythroblasts by α-toxin. (A) A total of 5 × 105 bone marrow cells were cultured for 1, 3, or 6 hours in the presence or absence (Control) of 100 ng/ml α-toxin (Wild-type) or a variant α-toxin (H148G), and flow cytometry analysis was performed. The frequency of annexinV+ cells in the TER119+ cell population was determined. (B) A total of 5 × 106 bone marrow cells were cultured for 24 hours in the presence of 10 ng/ml α-toxin (α-toxin) and 1 U/ml recombinant human EPO. The absolute number of TER119+ cells per culture well was determined by flow cytometry. One-way ANOVA was employed to assess statistical significance. Values are mean ± standard deviation. *P < 0.05.
