Figure 5
From: Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation
α-Toxin impairs differentiation of K562 cells. K562 cells were cultured in the presence of 30 μM hemin. Simultaneously, the cells were treated with 10 ng/ml α-toxin (Wild-type) or a variant α-toxin (H148G). (A,B) Hemoglobin synthesis in K562 cells was determined using a TMB assay. The relative amounts of hemoglobin are expressed as a percentage relative to the control cells. (C) The cell viability was evaluated using a CCK-8 assay. The relative cell viabilities are expressed as a percentage relative to α-toxin-untreated control cells. One-way ANOVA and two-tailed Student’s t-test were employed to assess statistical significance. Values are mean ± standard deviation. *P < 0.05; **P < 0.01; n.s., not significant.
