Figure 5
From: Investigation of brain tissue infiltration by medulloblastoma cells in an ex vivo model
Massive growth and brain tissue infiltration of group 3 MB cells. (A) LA-EGFP DAOY or HD-MBO3 cell spheroids were implanted in DIV 15 cerebellar slices and incubated for 5 days. Image show confocal microscopy image analysis of whole slices. Staining as indicated. Tumour area relative to whole cerebellar slice area was quantified (right panels) and was 2.9 and 29.0% for DAOY and HD-MBO3, respectively. (B) Growth of HD-MBO3 spheroid in OCSC and microscopy analysis as in (A). Inverted grey scale images show Vybrant Dil staining of the tumour cells. Graphical representation of the occupancy obtained from three independent experiments for each cell line. Means and SD are shown with statistical analysis done using unpaired, two-tailed t-test. (a) is a higher magnification confocal image of the tumour-parenchyma border. Yellow line in (b) indicates tumour border and yellow arrowheads EdU-positive nuclei of infiltrating tumour cells. (c) as (a) but with yellow arrows pointing to EdU-negative infiltrating tumour cells identified by Vybrant Dil staining (green). Asterisks indicate EdU-positive nuclei of infiltrating tumour cells.
