Figure 2
From: NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish
NEAP suppresses NGF-induced TrkA and FGFR1 phosphorylation. PC12-cDNA4, NEAP, or NEAP-C152S cells were treated with NGF (50 ng/ml) and the cells were collected at the indicated time points. (a) Levels of TrkA, pTrkA, FRS2, and pFRS2 were examined by immune-blotting assays using specific antibodies as indicated. (b) Levels of FGFR1 and pFGFR1 were examined by immune-blotting assays. The phospho-protein/protein ratio in the untreated PC12-cDNA4 cells was designed as 1. The results from two independent experiments were presented in Supplementary Materials.
