Figure 5 | Scientific Reports

Figure 5

From: Suppression of VAMP2 Alters Morphology of the Tegument and Affects Glucose uptake, Development and Reproduction of Schistosoma japonicum

Figure 5

Effect of SjVAMP2 suppression on glucose uptake. (A) Glucose uptake by schistosomes in different groups cultured in low-glucose media for 2, 4 and 6 day, was measured respectively. Data was from three independent experiments, and compared with the DEPC-treated groups (**P < 0.01, ***P < 0.001). (B) Confocal laser scanning microscopy observation of S. japonicum glucose uptake in different groups after siRNA treatment. 2-NBDG was used as a fluorescent probe to determine glucose uptake. Worms not treated with 2-NBDG were used as a negative sample to control for background fluorescence. (C) The signals of 2-NBDG were analyzed using imageJ software.

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