Table 2 Fitting parameters of the complexation model for the data obtained in the titration experiments

From: DOM-mediated membrane retention of fluoroquinolone as revealed by fluorescence quenching properties

 

K M (L/mg)

I ML

\({\boldsymbol{f}}{\boldsymbol{=}}{\boldsymbol{(}}{\bf{1}}{\boldsymbol{-}}\frac{{{\boldsymbol{I}}}_{{\boldsymbol{M}}{\boldsymbol{L}}}}{{{\boldsymbol{I}}}_{{\bf{0}}}}{\boldsymbol{)}}{\boldsymbol{\times }}{\bf{100}}{\boldsymbol{ \% }}\)

R 2

SRDOM

0.86

0.19

0.84

0.97

SRDOM + HA

2.8

0.24

0.92

0.98

HA

4.07

0.18

0.95

0.98

  1. Note: K M , I ML and f represent the conditional stability constant, limiting fluorescence intensity value below which the FI does not change and fraction of the fluorescence intensity that corresponds to the binding fluorescence, respectively.