Figure 1 | Scientific Reports

Figure 1

From: Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation

Figure 1

The CCS was mixed and incubated with anti-EPO beads in a 96-well filter plate. After washing, pure proteins were eluted in HCl and freeze dried. Glycans were prepared by PNGaseF release without additional denaturation, lactonization for stabilization and HILIC-SPE. MALDI-TOF-MS analysis in reflectron positive mode yielded high quality spectra which were automatically quantified with MassyTools.

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