Figure 2 | Scientific Reports

Figure 2

From: Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation

Figure 2

Representative MALDI-TOF-MS spectra of EPO N-glycans. (A) A spectrum of the EPO standard. It should be noted that the antenna isomerism indicated for the partially sialylated tri-antennary glycan also applies to all other tri-antennary structures. In addition, there may be minor contributions of bi-antennary structures with one LacNAc repeat. The tetra-antennary glycans have significant contributions of tri-antennary structures with one additional LacNAc repeat, as exemplified for the glycan with two NANA and 4 LacNAc units. (B) The major N-glycans detected for EPO purified from CCS were very similar to those of the EPO standard albeit in different relative abundances. 2× and 3× gives the incidence of the monosaccharide or antenna to the right or below. The brackets signify that the exact positions of substituents are unclear. The N-acetylneuraminic acids to the right of the vertical brackets may cap an antenna or LacNAc-repeat.

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