Figure 7 | Scientific Reports

Figure 7

From: Plasticity in the Oxidative Folding Pathway of the High Affinity Nerita Versicolor Carboxypeptidase Inhibitor (NvCI)

Figure 7

Stop/go folding of the major folding/unfolding intermediates of NvCI. Acid-trapped intermediates (I, IIb and IIab) were purified by RP-HPLC, freeze-dried, and dissolved in Tris-HCl (pH 8.4) to reinitiate the folding reaction either in the absence (b) or presence (a) of 1 mM GSSG. Folding intermediates were subsequently trapped with acid at different time points and analyzed by RP-HPLC. The chromatograms at 0 minutes (shown in a) were obtained after resuspending the lyophilized intermediates in TFA 1% as a control of purity and homogeneity of each species.

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