Table 2 The four information levels of multidimensional flow cytometric data.
Information level | DAMACY output |
|---|---|
(1) The multivariate (co)-expression of markers on single cells | PCA (loadings) |
(2) Aggregation of cells into populations with similar marker expression | PCA (scores) |
(3) Representation of cell populations in a specific individual | Histograms |
(4) Differentiation of this representation in specific clinical phenotypes | Leukocyte map based on OPLS-DA weights. |