Table 2 The four information levels of multidimensional flow cytometric data.

From: Novel data analysis method for multicolour flow cytometry links variability of multiple markers on single cells to a clinical phenotype

Information level

DAMACY output

(1) The multivariate (co)-expression of markers on single cells

PCA (loadings)

(2) Aggregation of cells into populations with similar marker expression

PCA (scores)

(3) Representation of cell populations in a specific individual

Histograms

(4) Differentiation of this representation in specific clinical phenotypes

Leukocyte map based on OPLS-DA weights.