Figure 1
From: Fibulin-4 deposition requires EMILIN-1 in the extracellular matrix of osteoblasts
Localization of EMILINs in cartilage and bone. (A) Confocal immunofluorescence microscopy showing EMILIN-1 and -2, together with fibrillin-1, and -2 localization in transverse sections from newborn mouse tail. Each protein showed a specific distribution pattern among the different tissues present in the tail. However, signals detected for EMILIN-1 and -2 and fibrillin fibers were consistently overlapping. The insets in the merged panels show a higher magnification of the annulus fibrosus, where EMILIN-1 and fibrillin-2 fibers show co-localization. Scale bar: 75 μm. (B) Sagittal section of newborn mouse skull, showing the localization of the three EMILIN proteins by immunofluorescence in calvarial bone. Note the presence of EMILIN-1 and EMILIN-3, but not EMILIN-2, positive fibers in the ECM that directly surround the bone (arrowheads). Scale bar, 75 μm. (C) Transmission electron microscopy analysis of newborn mouse trachea, following immunogold labeling for the three EMILIN proteins (black dots). EMILIN-1 and EMILIN-3, but not EMILIN-2, are abundantly distributed on microfibrils. Scale bar, 500 nm.
