Figure 1 | Scientific Reports

Figure 1

From: Metformin ameliorates the Phenotype Transition of Peritoneal Mesothelial Cells and Peritoneal Fibrosis via a modulation of Oxidative Stress

Figure 1

Effect of Metformin on TGF-β-induced EMT. TGF-β1 (1 ng/mL) induces the morphologic changes of HPMCs from a cuboidal, cobble-stone appearance to elongated, fibroblastoid morphology with decreased expression of ZO-1 and the acquisition of α-SMA expression in HPMCs. (B,C) Real-time PCR (B) and western blot analysis (C) reveal a decreased E-cadherin expression with an increase in α-SMA, collagen type I and fibronectin by TGF-β1. Metformin significantly ameliorates TGF-β1-induced EMT (A–C). Representative cell morphology and fluorescein immunocytochemistry for ZO-1 (green) and α-SMA (green) with nuclear DAPI staining (blue) at 48 hours of TGF-β1 with or without metformin (A). Quantitation bars for real-time PCR (B, n = 5) and representative western blots with quantitation bars (C, n = 6) are shown. *p < 0.05 vs. others, #p < 0.05 vs. TGF-β1.

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