Figure 9 | Scientific Reports

Figure 9

From: Metformin ameliorates the Phenotype Transition of Peritoneal Mesothelial Cells and Peritoneal Fibrosis via a modulation of Oxidative Stress

Figure 9

Effect of Metformin on Peritoneal Fibrosis and EMT in Animal Model of Peritoneal Dialysis. (A) Masson’s Trichrome-stained tissue of parietal peritoneum shows that peritoneal thickness in dialysis group (D) is significantly higher than those in control group, which is decreased in group D + M. Representative histology of parietal peritoneum is shown with quantitation bar of peritoneal thickness. Magnification ×200. Scale bar 100 μm. *p < 0.05 vs. others, #p < 0.05 vs. group D. (B) Representative fluorescent confocal image of peritoneum shows a decreased cytokeratin (green) and increased α-SMA staining (red) in mesothelial lining with an appearance of cytokeratin (+)/α-SMA (+) cells in mesothelial and submesothelial zone (arrows). Rats in group D + M demonstrates a restoration of cytokeratin (+) mesothelial cells with a decrease in cytokeratin (+)/α-SMA (+) cells in mesothelial and submesothelial area. Magnification ×640. Scale bar 10 μm. (C) Western blot analysis with quantitation bar shows the changes of E-cadherin and α-SMA in group D are ameliorated by metformin treatment. *p < 0.05 vs. others, #p < 0.05 vs. group D.

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