Table 1 The primer used in clone and RNA interference.
Gene name | Primer name | Primer sequence (5′–3′) | Primer Introduction |
|---|---|---|---|
CgCSAD1 | T7-26339f | GATCACTAATACGACTCACTATAGGGACAGTTTGGGAGGATGTTGGTT | Prepare the template for amplification of dsRNA |
CgCSAD1 | T7-26339r | GATCACTAATACGACTCACTATAGGGACTGAATGGACTTGTCGCCG | Prepare the template for amplification of dsRNA |
CgCSAD1 | 26339HEf | CACAAAAGAATCCACGAC | partial coding region of CgCSAD1 gene |
CgCSAD1 | 26339HEr | TCATTCCCTCCTTGACCT | partial coding region of CgCSAD1 gene |
CgCSAD1 | 26339-3′-628 | ATGTTCTGCCCTGGCGGTTCTATT | 3′RACE PCR for CgCSAD1 |
CgCSAD1 | 26339-3′-1529 | CAGCCTCAATGATGGTCGGTTAC | 3′RACE PCR for CgCSAD1 |
CgCSAD1 | 26339-5′-853 | GAATCATCTTTCCATTAGCATCAGTCT | 5′RACE PCR for CgCSAD1 |
CgCSAD1 | 26339-5′-452 | GGATGGGCAACTTTCACGCTGTA | 5′RACE PCR for CgCSAD1 |
CgCSAD2 | 22071HEf | CATCCCAAAGAGTTAGAGG | partial coding region of CgCSAD2 gene |
CgCSAD2 | 22071HEr | GAAGAAATTGACATGCTCC | partial coding region of CgCSAD2 gene |
CgCSAD2 | 22071-3′-319 | GCCTGGCTCACTGAGGTCCTGAATA | 3′RACE PCR for CgCSAD2 |
CgCSAD2 | 22071-3′-159 | GGGAGAGACTGGGAAAGGTGGCT | 3′RACE PCR for CgCSAD2 |
CgCSAD2 | 22071-5′-866 | CGGTATTTCTTGGACAACAAGG | 5′RACE PCR for CgCSAD2 |
CgCSAD2 | 22071-5′-312 | CAACCTGTAATCATCCAGTCCACT | 5′RACE PCR for CgCSAD2 |
