Figure 3
From: Cryopreservation protocol for human biliary tree stem/progenitors, hepatic and pancreatic precursors
Expression of pluripotency and multipotency genes in cultures of cryopreserved or freshly isolated hBTSCs under self-renewal (KM) or hormonally defined medium for multiple endodermal mature fates (hepatocytic/HM, cholangiocytic/CM, pancreatic islets/PM). (A) Relative gene expression of SOX2, EpCAM, OCT4, PDX1, SOX17, SOX2 in cryopreserved hBTSCs in Sol1 and in Sol3 (not shown) under different culture conditions. Previously cryopreserved hBTSCs cultured under self-renewal conditions in Kubota’s Medium (KM) reduced the expression of pluripotency and multipotency genes when transferred in hormonally defined medium for particular endodermal mature fates (hepatocytic/HM, cholangiocytic/CM, pancreatic islets/PM). Data are expressed as mean ± SD of 3 experiments; *p < 0.05; §p < 0.01; **p < 0.05 HM vs CM and PM; §§p < 0.05 PM vs CM and HM. (B) Relative gene expression of Nanog, SOX2, EpCAM, OCT4, PDX1, SOX17, SOX2 in freshly isolated (FI) hBTSCs cultured in different defined conditions. Freshly isolated hBTSCs cultured under self-renewal conditions in Kubota’s Medium (KM) reduced the expression of pluripotency and multipotency genes when transferred in hormonally defined medium for particular endodermal mature fates (hepatocytic/HM, cholangiocytic/CM, pancreatic islets/PM). Data are expressed as mean ± SD of3 experiments; *p < 0.05; §p < 0.01 **p < 0.05 HM vs CM and PM; §§p < 0.05 PM vs CM and HM.
