Figure 2
From: The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling
Hypoxia induces leptin production and secretion by human APCs. (a) Highly induced LEP protein levels in APC conditioned media collected under hypoxia as compared with normoxia. Data are medians and 5–95 percentiles from twenty-one APCs lines. ***p < 0.01. (b) LEP protein levels in conditioned media of BM-MNCs (n = 3, p = 0.09 vs. normoxia). (c) Time course of LEP transcription and LEP protein production and secretion under hypoxia and following return to normoxia in three individual APC lines. Data are mean and SEM. (d) Concomitant changes in LEPR mRNA levels in the same cell lines. (e) Representative immunoblots for the expression of LEPR protein in APCs under normoxia and hypoxia during 48 h. Band densitometry was determined using Image J software and the fold change was calculated per each APC line (n = 3). Data are shown as mean ± SEM and asterisk means statistical significance as per p = 0.049.
