Figure 1
From: A new approach for ratiometric in vivo calcium imaging of microglia
Expression of target genes (GFP or Twitch-2B) in LV.miR-9.T vector-transduced brain tissue. (a) Scheme of the LV.PGK.GFP.miR-9.T construct. (b) Maximum intensity projection (MIP) images (5–15 µm, step 1 µm) illustrating the identity of LV.PGK.GFP.miR-9.T-transduced cells (left) through immunofluorescent staining with an antibody against the microglial marker Iba1 (middle). The colour merged image is shown on the right. (c) Two-photon MIP in vivo image (18–27 µm, step 2 µm) of the transduced cells showing typical microglial morphology. Inset is a MIP image (3–21 µm, step 1 µm) showing a zoom-in of a rectangular square in the middle of the image. (d) Scheme of the LV.PGK.Twitch-2B.miR-9.T construct. (e,f) MIP images ((e) 0–19 µm, (f) 0–20 µm, step 1 µm) showing either native fluorescence of the cells transduced with LV.PGK.Twitch-2B.miR-9.T construct (left) or fluorescence of the same area labelled with an anti-GFP antibody recognizing Twitch-2B (right).
