Figure 4
From: A new approach for ratiometric in vivo calcium imaging of microglia
LV.CMV.Twitch-2B.miR-9.T vector enables ratiometric calcium imaging of microglia in vivo. (a) MIP images (left: 18–33 µm depth; right: 17–36 µm depth, step 1 µm) showing combined fluorescence of mCerulean3 and cpVenusCD of cortical microglia in vivo. (b) Representative traces, recorded from the region of interest delineated in the inset (dashed line), showing mCerulean3 (top) and cpVenusCD (middle) channels for three pressure applications of a P2Y receptor agonist UDP (1 mM in the application pipette, 200 ms). Inset: MIP image (117–153 µm depth, step 1 µm) of the recorded microglial cell. Bottom: trace showing the ΔR/R signal. Arrowheads indicate time points of UDP applications. Similar results were obtained in n = 5 cells (summarized in Fig. 5b).
