Table 1 Information from manufacturers kit manual and included in the kits.
From: Comparison of commercial ELISA assays for quantification of corticosterone in serum
DRG-5186 | DRG-4164 | Enzo | Arbor Assays | |
|---|---|---|---|---|
Standard series: | 15–2250 ng/ml. | 1.7–83 ng/ml | 0.032–20 ng/ml. | 0.078–10 ng/ml |
Analytical sensitivity: | 4.1 ng/ml | 0.5647 ng/ml | 0.02699 ng/ml | 0.0186 ng/ml |
Amount of sample (for dilution): | 20 µl | 10 µl | 10 µl | 5 µl |
Sample dilution: | 1:1 | 1:10 | 1:40 | 1:100 |
Release from CBG: | n.s. | n.s. | YES | YES |
Internal quality control | Yes* | Yes | No | No |
Cross reactivity (%): | ||||
Progesterone | 0.7 | 7.4 | 1.7 | 0.24 |
Aldosterone | 0.2 | n.a. | 0.18 | 0.62 |
Cortisol | 0.3 | 0.3 | 0.046 | 0.38 |
11-Deoxycorticosterone | 2.4 | 3.4 | 28.6 | 12.3 |
Dehydroepiandrosterone | n.d. | n.a. | n.a. | n.a. |
Estriol | n.d. | n.a. | n.a. | n.a. |
Estradiol | n.d. | n.a. | n.a. | <0.08 |
17-hydroxyprogesterone | n.d. | n.a. | n.a. | n.a. |
18-Hydroxydeoxycorticosterone | n.d. | n.a. | n.a. | n.a. |
Testosterone | n.d. | n.a. | 0.13 | n.a. |
Pregnolone Prognenolone | n.d. | 0.3 | <0.03 | n.a. |
11-Dehydrocorticosterone | n.a. | 1.6 | n.a. | n.a. |
Tetrahydrocorticosterone | n.a. | n.a. | 0.28 | 0.76 |
β-Estradiol | n.a. | n.a. | <0.03 | n.a. |
Cortisone | n.a. | n.a. | <0.03 | <0.08 |
11-dehydrocorticosterone acetate | n.a. | n.a. | <0.03 | n.a. |
Dexamethasone | n.a. | n.a. | n.a. | 0.12 |
Corticosterone-21-Hemisuccinate | n.a. | n.a. | n.a. | <0.1 |
Other steroids | n.a. | <0.1 | n.a. | n.a. |
