Figure 2
ATO-induced apoptosis in A549 and A549R cells. A549 and A549R cells were treated without or with 20 µM ATO for 24 hours. Apoptotic cells were detected by Annexin V-FITC/PI dual staining using flow cytometry, and the percentage of apoptotic cells was calculated. Cellular levels of apoptosis-associated protein Bax and Bcl-2 were detected by immunoblotting. (A) The flow cytometry profiles of apoptotic cells in A549 and A549R cells without or with ATO treatment. (B) The percentage of apoptotic cells in A549 and A549R cells under different treatments. (C,D) The expression of Bax and Bcl-2 proteins in A549 and A549R cells and “Fold change” of the cellular level of Bax and Bcl-2 proteins. The blots were cropped for the clarity of the results. Results were obtained from three independent experiments, and quantitative results are presented as mean ± standard deviation (SD). P < 0.05 was designated as statistical significance. “*” indicates a significant difference compared with corresponding control group (P < 0.05). “#” indicates a significant difference compared with A549 cells (P < 0.05).
