Figure 4

The level of miR-155 in A549 and A549R cells. The level of miR-155 in A549 and A549R cells as well as A549R cells transfected with the miR-155 mimic control (MC), miR-155 mimic (M), miR-155 inhibitor control (IC) and miR-155 inhibitor (I) was determined with real-time PCR. (A) The miR-155 level in A549, A549R, and A549R cells transfected with MC, M, IC and I. The “Fold change in the level of mi-R155” was calculated based on the miR-155 level in A549R cells. The results of cell colonies and the percentage of colony formation were illustrated in (B,C), respectively. (D,E) The image of migratory cells (100×) and the number of migrating cells of A549R cells. Results were obtained from three independent experiments, and the quantitative results are presented as mean ± standard deviation (SD). “*” indicates a significant difference compared with the cells transfected with MC and IC (P < 0.05).