Figure 3
From: Coronavirus nucleocapsid proteins assemble constitutively in high molecular oligomers
Multiple domains mediate N protein oligomerization. Bacterial extracts from E. coli expressing the 6xHis-tagged N1, N2a and N2b-N3 truncations were incubated with immobilized GST, GST-N protein, GST-N1, GST-N2a and GST-N2b-N3. Precipitated proteins were eluted in SDS-sample buffer and analyzed by western blot using the anti-6xHis monoclonal antibody. A260/280 ratios <0.1 indicated the absence of nucleic acids in the samples.
