Figure 1

Enterocyte-specific Raptor is required for maintaining tuft cell abundance and function. Vilin-Cre ^ER (−); Raptor (f/f) (WT, n = 6) and Vilin-Cre ^ER (+); Raptor (f/f) (i-Raptor−/−, n = 6) mice were injected intraperitoneally with Tamoxifen (TAM) for 3 consecutive days and small intestine removed 2 weeks later. (a) Representative immunohistochemistry (IHC) shows tuft cells in brown using anti-DCLK1 antibody. (b) Quantification of tuft cells based on at least 20 well orientated crypt-villi axis. (c) Representative dissociation curve shows RT-PCR amplification of 28S rRNA gene from Tritrichomonas muris (Tm) positive or negative feces. (d) Representative Western blot analysis of the protein expression as indicated. GAPDH was used as a loading control. (e) Real-time PCR measurements of cytokines expression in enterocytes as indicated. (f) Real-time PCR measurements of cytokines expression in lamina propria as indicated. (g) Tm burden quantified from stools collected before and 7 days after TAM injection. Mean ± SD is shown. Student’s t test (*p < 0.05).