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Figure 1

From: Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs

Figure 1

Ribosome profiling in adipocytes. (A) Schematic diagram of the experimental design. Primary brown and white preadipocytes were isolated from interscapular brown adipose (BAT) and inguinal white adipose (WAT), cultured in vitro for differentiation. Cells at day 0 and day 5 were harvested for RNA-seq and ribosome profiling in parallel. BAT and epididymal white adipose tissue (eWAT) were harvested for RNA-seq and ribosome profiling. (B) Metagene analysis of translation initiation and termination. Average ribosome footprint density profiles of all the detectable mRNAs in BAT are aligned at their start codon and stop codon. (C) Hierarchical clustering of all samples in this studies based on their mRNA expression and RPF. (D–G) GSEA analysis of ribosome profiling data during (D) brown and (E) white adipogenesis (D5/D0); (F) Cultured brown adipocyte vs. white adipocyte (BAT D5)/(WAT D5); (G) BAT vs WAT (BAT/WAT). (H) Examples of ribosome profiling data over two genes: Fabp4 in brown adipocyte culture Day 0 vs. Day 5; (I) Ucp1 in BAT vs WAT. (J) Correlation between RNA-seq and ribosome profiling data in WAT-Day0, BAT-Day0, WAT and BAT samples.

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