Figure 1

Global and gene-specific evaluation of augmented temporal resolution in mES differentiation. (A) Schematic of mouse embryonic stem cell (ESC) differentiation into embryoid bodies (EB) over the time course evaluated here. (B) Analysis of the top three principle components (PCs) based on the 2,000 most variable genes from biological duplicate-6 hourly transcriptomes and KEGG pathway enrichment for 500 genes contributing most to each of the top three PCs. (C) Expression profiles of genes associated with pluripotency, primitive streak formation and cell specialization.